Madras Diabetes Research Foundation
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Madras Diabetes Research Foundation is situated at No 4, Conran Smith Road, Gopalapuram, Chennai - 600 086 in Chennai.
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Madras Diabetes Research Foundation - MDRF
Madras Diabetes Research Foundation (MDRF) established in 1996 by Dr. V. Mohan, internationally acclaimed diabetologist and research scientist and his wife Late Dr. Rema Mohan, internationally known specialist in diabetic eye disorders. MDRF was founded with the vision of providing a world class environment for research in diabetes and its complications. Within its short span of existence, MDRF has built up strengths in basic, clinical and epidemiological research. The quality of its research in diabetes and its complications is evidenced by numerous original publications in reputed peer reviewed journals. The institute also collaborates with several international and national centres. A 100% non-profit organization; MDRF depends largely on private donors and government research support for its growth and activities. Its core departments have improved over the years with the addition of state-of-the-art facilities. The major strength of the institute lies in well-structured epidemiological studies that help the ongoing biochemical, genetic, molecular and cell-based studies. Another main area where we focus is the definition of individuals at risk of diabetes or its complications by identification of risk markers. Research is a long-term investment where the fruits will be visible only years later. However what is very gratifying is that our research has had an immediate impact on the community.
Why join Madras Diabetes Research Foundation
Well infrastructure with all required facilities.
1 Type Courses Available
- 1 phd Courses
Madras Diabetes Research Foundation Placements
The state-of-the-art animal facility at the MDRF enables breeding and experimentation on small animals (rats and mice) to meet the needs of the insititute’s different departments. The facility provides opportunities for pre-clinical safety studies for cell-based therapies, proteins and new chemical entities to be conducted on animals. Its mission is to maintain a healthy balance between animal ethics, welfare and experimentation. Experiments on animals are performed with the approval of the Scientific Advisory Committee (SAC) and Institutional Animal Ethics Committee (IAEC) of the Institute and the overall activites are under the supervision of Dr. M. Balasubramanyam.
The Animal Facility is registered with the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Government of India, and strictly conforms to its guidelines.
Salient Features of the Animal Facility
1. Barrier maintained facility (total built up area of 400 sq.ft.) with 3 animal rooms
2. Rodent breeding in individually ventilated cages (IVC) to prevent adventitious microbial agents
3. Different AHUs for ventilation to maintain area classification
4. Rotary sieve to ensure dust-free bedding material
5. Unique animal identification system
6. Periodic validation of all equipment
Specialized Animal Services
Development of animals models for human diseases particularly insulin resistance and type 2 diabetes.
Pre-clinical Research Services
MDRF plans to conduct the following pre-clinical safety studies in accordance with national and international guidelines, ensuring GLP compliance.
a) Single dose toxicity studies
b) Repeated dose toxicity studies
c) Sensitization studies
d) Safety handler studies
Confocal Microscope Facility
Our central instrumentation lab houses a state of the art Confocal microscope facility. Equipped with optimized, user friendly hardware and software, this system delivers excellent confocal images and image stacks, especially in fluorescence applications. The system is equipped to study intracellular localization, protein-protein interactions, FRET, FRAP and many more cell biology applications.
The MDRF has a fully equipped laboratory dedicated to gene cloning studies. The state of the art facility focuses on simple sub cloning to site-directed mutagenesis in bacterial and mammalian systems. The facility offers services including gene characterization, cloning and expression.
Central Instrumentation Facility
The central instrumentation facility is located in the basement of the MDRF. The facility is equipped with the newer and more sophisticated instruments pertinent to the area of cellular, molecular and biochemical research complementing the basic instruments integral to cellular, molecular and biochemical research.
Micro Array Core Facility
Micro array Core Facility is equipped with state-of-the-art molecular profiling technology to meet the demands of high-throughput SNP analysis and expression patterns of thousands of genes in parallel. Our goal is to apply innovative technology and revolutionary assays towards the analysis of the genetics of diabetes.
Sequenom MassARRAY Facility
MassARRAY Compact system Gene Amp PCR System 9700 MassARRAY Nanodispencer
Sequenom MassARRAY, a high throughput platform for SNP genotyping, DNA methylation and gene expression analysis.
The Radioisotope facility at the MDRF is equipped with a radioisotope hood, GM counter (ECI), Liquid scintillation counter (Beckmann), gel electrophoresis unit (Broviga), gel transfer unit (Broviga), Isotope storage facilities and disposal facilities. Presently, radioisotope laboratory caters extensively for end point readout assays such as glucose uptake, palmitate uptake, thymidine uptake, and glycogen synthase activity measurements. Apart from end-point read out arrays Radioimmunoassay (RIA) and Electrophoretic Mobility Shift Assay (EMSA) measurements are also done in the facility.
Tissue culture facility
The Cellular and Molecular Biology (CMB) department has a lab dedicated for tissue culture work. This facility serves as a training lab for tissue culture course work, tissue culture experiments carried out by students involved in research and faculty research projects. Much of the equipment in the tissue culture facility was procured through the FIST (Fund for Infrastructure in Science and Technology) grant awarded to Dr. M. Balasubramanyam in 2003. The lab was renovated in 2008 when the facility was moved to Siruseri.
The facility is equipped to grow mammalian cells. Equipment at the facility include flowhoods, culture incubators, cell counter, inverted phase contrast microscope, primary cultures and cultivation of established cell lines, and human tissues. The aim is to maintain the highest standards, to teach and assist students and researchers in both intra and inter departmental research from within our department and to facilitate collaborative research. The Cell/Tissue Culture Core provides investigators belonging to the institute and their team member with training and updates on the newest techniques in cell/ tissue culture, assistance with experimental design, and facilitating procurement of specific cell lines, organs and tissues. The Cell/Tissue Culture Core provides Core Investigators and coworkers with:
Basic training in culturing and studying cells and tissues
Training in cell cloning, preparation of irradiated feeder layers, suspension and reaggregate cultures
Strategic advice in planning experiments and determining their feasibility
Cell culture facilities and specialized equipment such as biohazard hoods needed for culture experiments with toxic agents,
Facilities to process human tissues
Target cell lines, especially primary cultures of myoblasts, retinal endothelial cells
Backup storage of valuable lines derived during this work,
Development of new cell, tissue culture techniques and
Direction to appropriate local experts for specialized consultations. In addition, the Cell/Tissue Culture Core serves as a seedbed for developing new ideas, matching collaborators and facilitating communication among its users.
The recent trend in medical research is the rise in awareness of the importance of biobanking facilities as prerequisites to gaining knowledge on the development of disease-preventing strategies. The biobanking facility at the MDRF is focused at networking with the foundation’s various departments in the study of the causal pathways involved in diabetes and its complications. The facility is equipped with 17 freezers [15 freezers at -80°C and 2 freezers at -20°C] to achieve this end. It mission is to adhere to the strictest norms governing biobanking. Hence; no sample is procured or stored without obtaining prior ethical clearance from the Institute’s Human Ethical Committee and the written consent of the individual kind enough to donate the sample. Currently; the facility has in storage approximately 75,000 blood samples, 10,000 serum samples, 16,000 genetic samples [whole blood samples and samples taken on filter paper] and 5,000 urine samples obtained from its population-based studies.
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