CBSE Class 12th Biology Chapter 11 - Biotechnology - Principles and Processes Important Questions with Answers

You should focus on solving CBSE Class 12th Biology Chapter 11: Biotechnology - Principles and Processes important questions, especially to help you score high marks. By solving CBSE Class 12th Biology 11 questions, you will be solving exam-oriented questions only.

Never Miss an Exam Update

Prepare thoroughly with the most important questions of CBSE Class 12th Biology Chapter 11 - Biotechnology - Principles and Processes. You can first cover the CBSE Class 12th Biology syllabus to understand the key topics and then start solving the CBSE Class 12th Biology Chapter 11 - Biotechnology - Principles and Processes Important Question to get a better understanding of your preparation level. Start practicing now.

Are you feeling lost and unsure about what career path to take after completing 12th standard?

Say goodbye to confusion and hello to a bright future!

Was this article helpful?

0 Upvotes

0 Downvotes

What is the cell that receives a recombinant gene called ? (All India 2019)

Question 2.

Write the specific point in the palindrome and the bond that is cut by Eco Rl. (All Indio 2019)

Question 3.

ß-galactosides enzyme is considered a better selectable marker. Justify the statement. (Delhi 2019)

Question 4.

Expand ‘BAC’ and ‘YAC’. What are they and what is the purpose for which they are used? (All India 2019)

Question 5.

Explain how ‘sticky ends’ are obtained in a DNA segment. Write their importance in DNA technology year. (All India 2019)

Question 6.

(i) Mention the importance of gel-electrophoresis in biotechnology.
(ii) Explain the process of this technique. (All India 2019)

Question 7.

How does p-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain. (All India 2019)

Question 8.

Give reason why
(i) DNA cannot pass into a host cell through the cell membrane.
(ii) Proteases are added during isolation of DNA for genetic engineering.
(iii) Single cloning site is preferred in a vector. (All India 2019)

Question 9.

How is a continuous culture system maintained in bioreactors and why? (Delhi 2019)

Question 10.

Describe the roles of (i) high temperature, (ii) primers and (iii) bacterium.
Thermus aquaticus in carrying the process of polymerase chain reaction. (All India 2019)

Question 1.

Describe the process of amplification of ‘gene of interest’ using PCR technique. (Delhi 2019)

Question 2.

Explain three steps involved in polymerase chain reaction.
(i) List the three steps involved in Polymerase Chain Reaction (PCR).
(ii) Name the source organism of Taq polymerase.
Explain the specific role of this enzyme in PCR. (2018C; Foreign 2014)

Question 3.

(i) How has the development of bioreactor helped in biotechnology?
(ii) Name the most commonly used bioreactor and describe its working. (2018)

Question 4.

Explain the role(s) of the following in biotechnology
(i) Restriction endonuclease
(ii) Gel-electrophoresis
(iii) Selectable markers in pBR322 (Delhi 2017)

Question 5.

(i) Explain the significance of palindromic nucleotide sequences in the formation of recombinant DNA.
(ii) Write the use of restriction endonuclease in the above process. (All India 2017)

Question 6.

Describe the roles of heat, primers and the bacterium Thermus aquaticus in the process of PCR. (All India 2017)

Question 7.

Write the steps you would suggest to be undertaken to obtain a foreign-gene product. (Delhi 2017)

Question 8.

Suggest a technique to a researcher who needs to separate fragments of DNA. (Delhi 2016)
Or
Mention the use of gel electrophoresis in biotechnology experiments. (Outside Delhi 2016C)

Question 9.

Name the technique that is used to alter the chemistry of genetic material (DNA, RNA) to obtain desired result. (Delhi 2016C)

Question 10.

Explain the mode of action of Eco RI. (Delhi 2016C)
Or
What is Eco RI? How does Eco RI differ from an exonuclease? (Delhi 2015C)
Or
How does a restriction nuclease function? Explain. (All India 2014)